(DOWNLOAD) "Molecular Cloning, Expression, Purification and Characterization of the Zebrafish Catechol-O-Methyltransferases" by Adnan Alazizi ~ Book PDF Kindle ePub Free

📘 Read Now     📥 Download

eBook details

• Title: Molecular Cloning, Expression, Purification and Characterization of the Zebrafish Catechol-O-Methyltransferases
• Author : Adnan Alazizi
• Release Date : January 18, 2013
• Genre: Medical,Books,Professional & Technical,Science & Nature,
• Pages : * pages
• Size : 5432 KB

Description

The zebrafish is emerging as an important animal model for biomedical research. This study represents part of an effort to establish the zebrafish as a model for investigating the methylation pathway of drug metabolism. Two zebrafish catechol O-methyltransferases (COMTs), designated COMT-1 and COMT-2, were cloned, expressed, purified, and characterized. Substrate specificity of these two zebrafish COMTs were analyzed using a panel of catechol drugs including dobutamine, methyldopa, and isoproterenol, as well as endogenous catecholestrogens, catecholamines, and dopa. The expression of COMT-1 was examined from embryogenesis to maturity by employing both the conventional RT-PCR and RT-quantitative PCR. Data on the COMT-1 expression provided insights into the developmental expression of the COMT enzyme, which may serve as a basis for further investigation into its possible protective role against the adverse effects of catechol drugsand other xenobiotic catechols during the developmental process.

Download Books "Molecular Cloning, Expression, Purification and Characterization of the Zebrafish Catechol-O-Methyltransferases" PDF ePub Kindle